Aim: The aim of this study was to examine the relationship between food literacy, health literacy, and healthy eating obsession in call center employees. Material and Methods: The cross-sectional descriptive study was conducted with 545 participants working in a call center, of whom 68.1% (n=371) were female and 31.9% (n=174) were male. A questionnaire consisting of sociodemographic data form, and perceived food literacy, health literacy, and ORTO-R scales were applied to the participants. Results: While 51.7% (n=282) of the participants had a normal body mass index, 41.7% (n=227) were overweight. Perceived food literacy (p=0.008) and ORTO-R (p=0.004) scores of female participants were higher than male participants, and those who were married had higher perceived food literacy scores than singles (p=0.003). Underweight individuals scored higher perceived food literacy than normal-weight individuals, and normal-weight individuals scored higher than overweight individuals (p=0.004). ORTO-R scores of the overweight group were higher than the other two groups and the difference was statistically significant (p<0.001). According to the regression analysis, health literacy decreased by 5.026 units for those who do not think they are eating healthy, and by 9.943 units for those who do not know how a healthy diet should be. Overweight participants exhibit more orthorexic eating behavior (p<0.001). Conclusion: Effective and continuous training programs will be beneficial for call center employees who have inactive and sedentary working conditions due to their work, in order to determine their deficiencies or inadequacies in terms of gaining food literacy, health literacy, and healthy eating behavior.